3D model to investigate mechanisms of cancer cell invasion
2017
PSG College of Technology, Coimbatore, India
Breast cancer metastasis is a complex process and current models poorly reproduce it in vitro. This hinders the investigation of the mechanisms underlying it and the development of therapeutic strategies. Thus, better in vitro models are needed, and 3D platforms have had a growing interest in recent years. Here, a gelatine nanofiber matrix was biofabricated and coated with collagen to mimic connective tissues and be used as a platform to culture human breast cancer cells. The researchers performed a physical characterization of the nanofiber matrix and confirmed its biocompatibility to culture human breast cancer cells. Furthermore, estrogen and progesterone were able to induce epithelial-to-mesenchymal transition and mesenchymal-to-epithelial transition, respectively, and increased the expression of metastasis-related genes. Additionally, this was confirmed by the enhanced invasive behaviour of cancer cells. Overall, in this study, a new biocompatible 3D platform is presented to investigate the mechanisms underlying cancer cell invasion in vitro.
An in vitro 3D model using collagen coated gelatin nanofibers for studying breast cancer metastasis
C Sabarinath
Added on: 10-26-2021
[1] https://iopscience.iop.org/article/10.1088/1758-5090/aa5510[2] https://data.jrc.ec.europa.eu/dataset/ffebe454-ed9a-47cf-8a33-8cf70c1b7d38
