miRNA-29 promotes invasive behavior in human breast cancer cells
2014
University of Alabama at Birmingham, Birmingham, USA
N-Myc Interactor, whose expression is affected in advanced stages of breast cancer, is involved in maintaining epithelial phenotype. Its downregulation can induce a metastatic phenotype of cancer cells in breast tumors. Thus, it is potentially involved in several critical processes in breast cancer development and metastasis. Here, 2D and 3D culture models with human breast cancer cell lines and patients' samples were used to investigate miRNAs that can potentially regulate N-Myc Interactor's expression and promote metastatic behaviour in cancer cells. The results show that miR-29 is more expressed in cancer cells that underwent the epithelial-to-mesenchymal transition. Moreover, in 2D and 3D cultures, miR-29 promoted a mesenchymal cell-like morphology. Furthermore, the modulation of miR-29 in different cell lines with high and low expression of N-Myc Interactor resulted in a negative correlation with N-Myc Interactor expression. Additionally, this was confirmed in samples from patients. Finally, the mechanism responsible for the upregulation of miR-29 expression in the absence of the N-Myc Interactor was elucidated. Overall, the researchers demonstrate a new potential therapeutical target that develops a positive regulatory loop that enhances miR-29 expression through the downregulation of the N-Myc Interactor, which promotes epithelial-to-mesenchymal transition and leads to tumor invasive behavior.
microRNA-29 negatively regulates EMT regulator N-myc interactor in breast cancer
Rajeev S Samant
Added on: 10-25-2021
[1] https://molecular-cancer.biomedcentral.com/articles/10.1186/1476-4598-13-200[2] https://data.jrc.ec.europa.eu/dataset/ffebe454-ed9a-47cf-8a33-8cf70c1b7d38
