Human ex vivo skin model to elucidate autoantibodies' role in breaking intercellular connections
December 2016
Toho University School of Medicine, Tokyo, Japan
Pemphigus is an autoimmune blistering disease of the skin and/or mucous membranes characterized by the loss of intercellular adhesion. In the present study, the researchers aimed at investigating the role of antibodies targeted at proteins relevant for the cell-to-cell interaction in blister formation. The researchers used ex vivo human skin samples from surgery leftover, treated them with antibodies and analyzed them by microscopy. Also, the skin samples properties were tested using an in vitro dissociation assay. The data suggest that a mixture of different antibodies can contribute to blister formation in PF. The study sheds light on the molecular mechanisms responsible for the skin blister and might identify potential targets for therapy development.
Non-pathogenic pemphigus foliaceus (PF) IgG acts synergistically with a directly pathogenic PF IgG to increase blistering by p38MAPK-dependent desmoglein 1 clustering
Ken Ishii
Added on: 10-19-2021
[1] https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5510496/[2] https://data.jrc.ec.europa.eu/dataset/700397b2-edd7-4ed6-86f7-fc1b164ed432
