Ex vivo model of autoimmune skin disorder used to reveal interplay of immune cells
December 2017
University of Bern, Bern, Switzerland
Bullous pemphigoid (BP) is the most common autoimmune subepidermal blistering disorder in the western world. Evidence shows the presence and interplay of different inflammatory cells, corresponding chemokines and mediators which induces and allows the progression of BP. In the present study, the researchers established an ex vivo skin model of BP to study the interplay and how mechanisms could be potentially used for the development of therapies. Skin sections were obtained from patients and incubated with human purified immune cells in the presence of BP autoantibodies. The sections were then characterized using various assays to assess the production of reactive-oxygen species, potential dermal-epidermal separation and release of factors. The results showed that the interaction of two specific types of immune cells was enhancing blister formation. The authors conclude that blocking intercellular cross-talk could promise a new therapeutic approach for blocking tissue damage in BP.
Monocytes enhance neutrophil-induced blister formation in an ex vivo model of bullous pemphigoid
Hans-Uwe Simon
Added on: 10-18-2021
[1] https://onlinelibrary.wiley.com/doi/10.1111/all.13376[2] https://data.jrc.ec.europa.eu/dataset/700397b2-edd7-4ed6-86f7-fc1b164ed432
