Tumour spheroids for drug screening
2015
Korea University, Seoul, South Korea(1)
Sogang University, Seoul, South Korea(2)
Sogang University, Seoul, South Korea(2)
Nanotechnology has arisen as a powerful tool to develop targeted therapies in cancer. However, the lack of reliable in vitro models limits the development of this technology. Here, human breast adenocarcinoma cells were cultured in 3D spheroids in arrays of concave microwells to test doxorubicin-loaded nanoparticles. The results showed that the loaded nanoparticles disrupted the small tumour spheroids, decreasing their size, but were ineffective against larger tumour spheroids. Moreover, those spheroids treated with doxorubicin-loaded nanoparticles were unable to preserve the circular shape. Finally, it was found that larger tumours had higher cell viability upon treatment and that the development of tight cell-cell junctions improved their drug resistance. Overall, the researchers present a new platform to model tumours in a 3D context where they develop resistance mechanisms potentially present in vivo. This opens the door to improved drug screening in cancer therapy.
Concave microwell array-mediated three-dimensional tumor model for screening anticancer drug-loaded nanoparticles
Sang-Hoon Lee(1), Bong Geun Chung(2)
Added on: 10-14-2021
[1] https://www.sciencedirect.com/science/article/abs/pii/S1549963415000556[2] https://data.jrc.ec.europa.eu/dataset/ffebe454-ed9a-47cf-8a33-8cf70c1b7d38
