Organotypic model for cancer drug screening
2015
Instituto Mexicano del Seguro Social, Monterrey, Mexico
Breast cancer is the most prevalent cause of cancer-related death in women worldwide. Despite the recent advances, there is still an urge to find efficient antitumour compounds with fewer adverse effects. Thus, better translational models are needed to perform more accurate drug screening and testing assays. Here, a new method is established to generate organotypic cultures of human breast tumour slices to assess the antineoplastic potential of several natural compounds. The results showed that the explants could be successfully cultured. Additionally, drug tests showed that paclitaxel alone could decrease explant viability, while natural compounds showed diverse effects. However, when combining paclitaxel with natural compounds, there was a synergistic effect of the drugs. Overall, the researchers establish a new methodology to perform an organotypic culture of human breast tumour slices to test ex vivo antitumour compounds.
Organotypic culture of breast tumor explants as a multicellular system for the screening of natural compounds with antineoplastic potential
Pilar Carranza-Rosales
Added on: 10-13-2021
[1] https://www.hindawi.com/journals/bmri/2015/618021/[2] https://data.jrc.ec.europa.eu/dataset/ffebe454-ed9a-47cf-8a33-8cf70c1b7d38
