Biochip to diagnose Alzheimer's disease
2014
Medical University of Vienna, Vienna, Austria
Alzheimer's disease is a multifactorial disorder that leads to brain degeneration, provoking cognitive deficits and, ultimately, death. The causes of the disease are not yet clear and the only diagnostic tools available are neuropsychological tests and brain imaging. Recently, new diagnostic tests based on markers in cerebrospinal fluid have been proposed, but they require invasive approaches. Here, platelet lysates of different groups of patients with neurodegenerative diseases were analyzed using a fluorescence two-dimensional differential gel electrophoresis in independent discovery and verification sets to identify biomarkers of Alzheimer's disease related to platelets. The results show that it was possible to efficiently discriminate Alzheimer's patients through a sum score of four identified proteins. Afterwards, the researchers developed a high-throughput multiplex protein biochip to apply the described profile for the diagnosis of Alzheimer's disease. The method developed in this study allows to rapidly screen samples of potential patients of Alzheimer's disease in a non-invasive approach through the detection of newly proposed biomarkers in a high-throughput system.
A platelet protein biochip rapidly detects an Alzheimer’s disease-specific phenotype
Maria Zellner
Added on: 09-30-2021
[1] https://link.springer.com/article/10.1007/s00401-014-1341-8[2] https://data.jrc.ec.europa.eu/dataset/a8fd26ef-b113-47ab-92ba-fd2be449c7eb
