Tissue engineering and in silico method for the study of cartilage degradation
2020
Charité – Universitätsmedizin Berlin, Berlin, Germany
This study aimed to combine in vitro and in silico modelling based on a tissue-engineering approach using mesenchymal condensation to mimic cytokine-induced cellular and matrix-related changes during cartilage degradation. Scaffold-free cartilage-like constructs (SFCCs) were produced based on self-organizing mesenchymal stromal cells (mesenchymal condensation) and characterized regarding their cellular and matrix composition. SFCCs were treated with interleukin-1β (IL–1β) and tumor necrosis factor α (TNFα) for 3 weeks to simulate OA-related matrix degradation. In addition, an existing mathematical model based on partial differential equations was optimized and transferred to the basic settings to simulate the distribution of IL–1β, type II collagen degradation and cell number reduction. By combining in vitro and in silico methods, the authors aimed to develop a valid, efficient alternative approach to examine and predict disease progression and the effects of new therapeutics.
Macroscale mesenchymal condensation to study cytokine-driven cellular and matrix-related changes during cartilage degradation
Annemarie Lang
Added on: 09-28-2021
[1] https://iopscience.iop.org/article/10.1088/1758-5090/aba08f
