Modulation of tyrosine hydroxylase expression for therapeutical strategies
2016
Iran University of Medical Sciences, Tehran, Iran
Tyrosine hydroxylase dynamics have been studied in Alzheimer's and Parkinson's disease and schizophrenia patients for some time now. The decrease in its levels influences catecholamine synthesis and this shows to be impaired in several neurological disorders. Here, the effects on tyrosine hydroxilase expression of noggin and different self-assembling nanofibers are investigated in neuron-like cells derived from human endometrial-derived stromal cells. The results show that BMHP-1 and laminin nanofibers induced higher cell viability through Wnt/beta-catenin signaling pathway, as assessed by Bcl2 and beta-tubulin III gene expression in neuroblastoma and neuron-like cells. While in other nanofibers other genes were induced, only in the laminin encapsulated cells in 2D cell culture with noggin-rich media was possible to observe overexpression of tyrosine hydroxylase. Overall, the researchers propose a new methodology to increase the expression of tyrosine hydroxylase through the encapsulation and treatment of induced neurons as a therapeutical strategy in different neurological disorders.
Noggin along with a self-assembling peptide nanofiber containing long motif of laminin induces tyrosine hydroxylase gene expression
Shima Tavakol
Added on: 08-31-2021
[1] https://link.springer.com/article/10.1007%2Fs12035-016-0006-0[2] https://data.jrc.ec.europa.eu/dataset/a8fd26ef-b113-47ab-92ba-fd2be449c7eb
