Neuronal cell mechanisms against toxic exposure
2018
Johns Hopkins Bloomberg School of Public Health, Baltimore, USA
Toxicologic studies performed in vitro often rely on exposure to tested compounds at high concentrations for short times. But these conditions are not those found usually in real-life exposures, which are usually at low doses for long term periods. Here, an in vitro 3D model of human dopaminergic cells is used to study the exposure to rotenone and study the acute response and the long-term adaptations in cells. When exposed to the toxic compound, dopaminergic cells underwent several metabolic and morphologic changes. After removal, some of the normal characteristics of these cells were recovered and this was reflected in transcriptomic changes present in the acute response that were not present one week after the wash-out of the rotenone. Moreover, pre-exposed cells showed different transcriptomic profiles and metabolic patterns to those that had not been exposed before, showing long-term adaptive behaviour of these cells. In summary, this study proposes a 3D in vitro neuronal model to perform toxicological studies that take into account the long-term mechanisms derived from continued exposure to toxic compounds.
Toxicity, recovery, and resilience in a 3D dopaminergic neuronal in vitro model exposed to rotenone
Lena Smirnova
Added on: 08-31-2021
[1] https://link.springer.com/article/10.1007/s00204-018-2250-8[2] https://data.jrc.ec.europa.eu/dataset/a8fd26ef-b113-47ab-92ba-fd2be449c7eb
