Comparative proteomic analysis of an in vitro 3D model with human brain samples
2018
Edith Nourse Rogers Memorial Veterans Hospital, Bedford, USA
Pluripotent stem cells are becoming a powerful tool to model neurodegenerative diseases. Despite this, the developed models are not well characterized, and this limits their application. In this study, a comparative proteomic analysis is done between 3D human neural spheroids and post-mortem brains from Alzheimer's disease patients. The neural spheroids are produced with human iPSC obtained from samples of human peripheral blood mononuclear cells. The results reveal that 3D neuro-spheroids from Alzheimer's patients have alterations in proteins related to axonal growth, mitochondrial function and antioxidant mechanisms. Similarly, post-mortem brains analysis shows alteration of proteins related to oxidative stress, neuroinflammation and proteins related to axonal injury. The researchers are able to show that the pathologic proteomic profiles generated in the "in vitro" model correspond to those found in post-mortem samples. Overall, this study confirms the potential of human iPSC-derived brain organoids for the study of neurodegenerative diseases and proposes a series of altered proteins that can be further studied to understand the pathophysiology of Alzheimer's disease.
Common proteomic profiles of induced pluripotent stem cell-derived three-dimensional neurons and brain tissue from Alzheimer patients
Weiming Xia
Added on: 08-19-2021
[1] https://www.sciencedirect.com/science/article/abs/pii/S187439191830191X[2] https://data.jrc.ec.europa.eu/dataset/a8fd26ef-b113-47ab-92ba-fd2be449c7eb
