Metabolic changes in vitro after protein aggregates challenge
October 2017
University of Liverpool, Liverpool, United Kingdom
Protein aggregation is a pathogenic process present in several neurodegenerative diseases. However, the aggregation states and their effects on cellular viability are unclear. Here, a method is developed to perform metabolomics using nuclear magnetic resonance to study the effects of different protein aggregates in a human neuroblastoma cell line. The results elucidate key metabolites related to different pathways that are altered in this cell line when challenged with different aggregated forms. Furthermore, the system is capable of suggesting different cellular behaviours for different aggregated forms. Monomer or oligomeric forms induce a cellular response against oxidative stress, while metabolic changes in fibrillar challenge are less prevalent but indicate a more toxic response driving to cytotoxicity. The researchers present a methodology that can be used to systematically study potentially toxic insults in different cell types to build metabolic profiles. This represents a potential step forward towards the identification of target and therapeutic strategies to alleviate the toxicity of protein aggregation in different neurodegenerative diseases.
Using an NMR metabolomics approach to investigate the pathogenicity of amyloid-beta and alpha-synuclein
Jillian Madine
Added on: 08-17-2021
[1] https://link.springer.com/article/10.1007%2Fs11306-017-1289-5[2] https://data.jrc.ec.europa.eu/dataset/a8fd26ef-b113-47ab-92ba-fd2be449c7eb
