Activity-dependent distribution of S6K1 in a cancer 3D model
2018
National Academy of Sciences of Ukraine, Kyiv, Ukraine
A spheroid model with a human breast adenocarcinoma cell line was used to study the importance of motility in the subcellular localization of the ribosomal protein S6K1. When in dense 2D cultures, S6K1 was localized in the cytoplasm, while it was translocated into the nucleus during migration assays. Meanwhile, in human samples, this protein was localized in the nucleus in cancerous tissues but in the cytoplasm in healthy controls. This study shows the dynamic possibilities that the combination of human samples and "in vitro" 3D models can bring to deciphering the mechanisms that drive cancer cells behaviour.
Nucleocytoplasmic distribution of S6K1 depends on the density and motility of MCF-7 cells in vitro
Antonina Khoruzhenko
Added on: 08-02-2021
[1] https://f1000research.com/articles/7-1332[2] https://data.jrc.ec.europa.eu/dataset/ffebe454-ed9a-47cf-8a33-8cf70c1b7d38
