3D model to study tumor resistance mechanisms
2015
University of Buenos Aires, Buenos Aires, Argentina
A new model based on culturing a HER2 overexpressing human mammary adenocarcinoma cell line as spheroids is used in this study to understand the role of tumor self-organization in Trastuzumab resistance. Compared to monolayer cultures, the efficacy of Trastuzumab was greatly reduced in the spheroids, as confirmed by the reduction in apoptosis. This was linked to an induction of autophagy, seen to be protective. Thus, this model helped to elicit key insights of drug resistance in tumor cells, showing that it can be a valuable tool to study antitumor therapies and improve their efficacy.
Autophagy protects from Trastuzumab-induced cytotoxicity in HER2 overexpressing breast tumor spheroids
Gabriel L Fiszman
Added on: 08-02-2021
[1] https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0137920[2] https://data.jrc.ec.europa.eu/dataset/ffebe454-ed9a-47cf-8a33-8cf70c1b7d38
