Method to isolate cancer stem cells
December 2016
Kermanshah University of Medical Sciences, Kermanshah, Iran
In this paper, it is shown that by using vincristine for 72 hours together with a suspension culture of human breast cancer cells is possible to obtain a highly pure culture of cancer stem cells. This is confirmed by immunofluorescence and gene expression of specific cancer stem cell genes. Also, the mammosphere formation assay shows an increased capacity of these isolated cells to form units. Therefore, the use of vincristine combined with cell suspension could be used as a method to isolate cancer stem cells.
Isolation of breast cancer stem cell from MDA-MB231 cell line using vincristine
Mozafar Khazaei
Added on: 07-31-2021
[1] https://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022016000400003&lng=en&nrm=iso&tlng=en#back[2] https://data.jrc.ec.europa.eu/dataset/ffebe454-ed9a-47cf-8a33-8cf70c1b7d38
