In vitro testing of engineered T cells to increase tumor homing
December 2017
Herlev University Hospital, Herlev, Denmark
One of the major obstacles for successful adoptive cell therapy (ACT), is the limited homing of effector T cells to immune-suppressive tumor sites. In the present study, the researchers aimed at equipping T cells with chemokine receptors matching the chemokines of the tumor microenvironment to improve homing. Using flow cytometry, T cells were analysed from malignant ascites and blood donors (ovarian cancer patients and healthy individuals). The researchers characterized the chemokine profile in ascites chemokines and the expression of corresponding receptors on circulating T cells and tumor ascites lymphocytes. Chemokine receptor was then transduced and showed increased migration towards tumors in vitro. This proof of concept study shows that chemokine receptor engineering is feasible and improves homing of transduced T cells towards the tumor microenvironment.
Improved migration of tumor ascites lymphocytes to ovarian cancer microenvironment by CXCR2 transduction
Manja Idorn
Added on: 07-29-2021
[1] https://www.tandfonline.com/doi/full/10.1080/2162402X.2017.1412029[2] https://data.jrc.ec.europa.eu/dataset/352f7dfd-05cf-434b-a96a-7e270dc76573
