Generation of novel immunotoxin to treat blood cancer via phage display
2015
Justus-Liebig-University Giessen, Giessen, Germany(1)
University Hospital RWTH Aachen, Aachen, Germany(2)
University Hospital RWTH Aachen, Aachen, Germany(2)
The current chemotherapy treatment for acute myeloid leukaemia (AML) has several drawbacks. More specific and efficient approaches are therefore required to eradicate completely malignant cells while leaving healthy cells unaffected. In this study, the researchers generated antibodies that bind specifically to the surface of AML blast cells and AML bone marrow biopsy specimens. The antibodies were isolated from an available library by phage display and tested in vitro for internalization. Engineering the selected antibody into an immunotoxin, it was tested on a leukaemia cell line for control of cell proliferation and the induction of apoptosis. This method may therefore be useful for the selection of novel disease-specific internalizing antibody fragments, providing a novel immunotherapeutic strategy for the treatment of AML patients.
Phage display-based generation of novel internalizing antibody fragments for immunotoxin-based treatment of acute myeloid leukemia
Mehmet Kemal Tur(1), Stefan Barth(2)
Added on: 07-28-2021
[1] https://www.tandfonline.com/doi/full/10.1080/19420862.2015.1007818[2] https://data.jrc.ec.europa.eu/dataset/352f7dfd-05cf-434b-a96a-7e270dc76573
