Non Animal Testing Database

COVID-19: Human 3D model reveals new targets to prevent severe disease

Institute of Hygiene and Medical Microbiology, Innsbruck, Austria
The authors used highly differentiated primary human 3D tissue models infected with SARS-CoV-2 patient isolates. Viral load, viral infectivity, intracellular complement activation, inflammatory mechanisms, and tissue destruction were analyzed by real-time RT-PCR, high content screening, plaque assays, luminex analyses, and transepithelial electrical resistance measurements. The authors showed that primary normal human bronchial and small airway epithelial cells respond to SARS-CoV-2 infection by an inflated local complement component 3 (C3) mobilization. They illustrated that targeting the anaphylatoxin receptors C3a receptor and C5a receptor in nonimmune respiratory cells can prevent intrinsic lung inflammation and tissue damage. This opens up a possibility for the treatment of COVID-19.
C5aR inhibition of nonimmune cells suppresses inflammation and maintains epithelial integrity in SARS-CoV-2–infected primary human airway epithelia
Doris Wilflingseder, Wilfried Posch
Added on: 06-21-2021
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