ToxCast ER Pathway Model for endocrine disruptors
Regulatory accepted 2016
European Union Reference Laboratory for Alternatives to Animal Testing, Ispra, Italy
The ToxCast estrogen receptor (ER) pathway model is a mathematical model that combines the results from 18 high-throughput screening (HTS) assays from the ToxCast and Tox21 research programs. The HTS assays measure ER binding, dimerization, chromatin binding, transcriptional activation and ER-dependent cell proliferation. The model uses activity patterns across the in vitro assays to predict whether a chemical is an ER agonist or antagonist, or is otherwise influencing the assays through a manner dependent on the physics and chemistry of the technology platform (“assay interference”). The output of the model provides an area under the curve (AUC) value for the potential of a chemical to cause ER agonism, normalized with respect to the positive control chemical, estradiol. Validated and regulatory accepted under TM2016-08 (US) by EURL ECVAM (TSAR list).
The ToxCast Estrogen Receptor Agonist Pathway Model
EURL ECVAM
Added on: 07-03-2020
[1] https://tsar.jrc.ec.europa.eu/test-method/tm2016-08
