KeratinoSens assay for the testing of skin sensitizers
Regulatory accepted 2010
European Union Reference Laboratory for Alternatives to Animal Testing, Ispra, Italy
The KeratinoSens™ test method supports discrimination between sensitizing and non-sensitizing chemicals. The activation of a signalling pathway, which occurs during a key event of the Adverse Outcome Pathway (AOP) of skin sensitization, is assessed.
The KeratinoSens™ is performed with an immortalized, adherent human HaCaT keratinocyte cell line containing a plasmid with the luciferase gene. Quantitative optical measurement is used to indicate small electrophilic substances that may act as skin sensitizers.
Validated and regulatory approved under TM2010-03 (EU) by EURL ECVAM (TSAR list), OECD Test No. 442D.
KeratinoSens assay for the testing of skin sensitizers
EURL ECVAM
Added on: 07-03-2020
[1] https://tsar.jrc.ec.europa.eu/test-method/tm2010-03[2] https://www.oecd-ilibrary.org/environment/test-no-442d-in-vitro-skin-sensitisation_9789264229822-en
