Dynamic cerebral organoids culture for developmental neurotoxicity
CompanyOctober 2024
University of North Texas, Denton, USA
Human brain organoids are a promising tool for assessing chemical effects on brain development, but traditional culture systems face challenges such as low throughput, poor reproducibility, insufficient maturity, and necrotic core formation. This study addressed these issues by using a dynamic pillar/perfusion plate system to culture cerebral organoids with improved proliferation and maturity through bidirectional flow. Organoids were exposed to ascorbic acid (non-toxic) and methylmercury (toxic) to evaluate developmental neurotoxicity (DNT). Ascorbic acid caused no changes, while methylmercury led to significant alterations in morphology and neural gene expression under dynamic conditions. Notably, methylmercury showed no adverse effects in static cultures, emphasizing the value of dynamic systems for DNT assessment. The pillar/perfusion plate system described in the study is commercially available from Bioprinting Laboratories Inc.
Dynamic culture of cerebral organoids using a pillar/perfusion plate for the assessment of developmental neurotoxicity
Moo-Yeal Lee
Added on: 02-11-2025
[1] https://iopscience.iop.org/article/10.1088/1758-5090/ad867e[2] https://3dbpl.com/
