Automatic generation and analysis of engineered human myocardium
2020
University Medical Center Göttingen, Goettingen, Germany
In the present study, a protocol for automatic generation, maturation and analysis of artificial human myocardium (EHM) is presented. A multi-tier plate enables the parallel long-term cultivation of up to 48 EHM models from pluripotent stem cells (PSC)-derived cardiomyocytes and fibroblasts. This plate can be included in a fully automated screening process that enables video-optical monitoring and analysis of contraction force and heart rate. The method is therefore suitable for carrying out different test series at the same time, or for pursuing different approaches in drug and disease studies. In summary, the protocol-generated EHM models can help accelerate time-consuming and complex studies, explore pathological mechanisms in greater depth, and identify potential drug candidates for drug development. The method has so far been successfully applied to human embryonic stem cells (HES) and iPSC-derived cardiomyocytes, subtype-specific, i.e. atrial and ventricular, and commercially available cardiomyocyte preparations were used.
Generation of engineered human myocardium in a multi-well format
Malte Tiburcy, Wolfram-Hubertus Zimmermann
Added on: 04-11-2024
[1] https://www.sciencedirect.com/science/article/pii/S2666166720300198?via%3Dihub[2] https://pharmacology.umg.eu/research/meyer-lab/
