Membrane matrix model of transparent cornea for chemical safety testing
Validated Method CompanyXCellR8, Daresbury, United Kingdom
The company XCellR8 offers the Ocular Irritection® test to identify and evaluate substances that are harmful to the eyes. The method allows the subdivision into non-irritating substances and substances that induce serious clouding of the cornea. In a first step, the compatibility of the test substance is checked by determining the pH value and the surfactant properties. The main test system consists of two components: a macromolecular matrix that simulates the transparent cornea and a membrane disk that enables controlled delivery of the test chemical to the matrix reagent. Depending on the biochemical properties, the substances are applied to the model membrane or directly to the gel matrix. After 24 hours, the optical density of the cornea model is quantitatively determined using a spectrophotometer. Based on the measured values determined in this way, a so-called Irritection® Draize Equivalent Score (IDE) is calculated for each tested substance, from which a Maximum Qualified Score (MQS) is derived. So far, it has been shown that the MQS has proven to be a reliable predictive value for substance classification according to the UN-GHS specifications. Validated and regulatory recognized under OECD Test No. 496. The method is also suitable as an effective step in pursuing top-down or bottom-up approaches following OECD guidance document 263 (Integrated Approaches to Testing and Assessment [IATA] for Serious Eye Damage and Eye Irritation).
Regulatory Ocular Irritection® test
info@x-cellr8.com
Added on: 02-29-2024
[1] https://www.oecd.org/environment/test-no-496-in-vitro-macromolecular-test-method-for-identifying-chemicals-inducing-serious-eye-damage-and-chemicals-not-requiring-970e5cd9-en.htm[2] https://x-cellr8.com/regulatory-safety-testing/ocular-irritection/
