Investigation of endocrine disrupting effects in avian cells
2015
Örebro University, Örebro, Sweden
Increased exposure of birds to endocrine disrupting compounds (EDC) can result in developmental and reproductive dysfunctions. The present study aimed to determine whether two flame retardants and a metabolite of a third flame retardant can interact with the chicken androgen receptor (AR).
In silico modelling studies showed that the investigated compounds were able to dock into the chicken AR ligand-binding pocket. In vitro assays using a chicken hepatocellular carcinoma cell line revealed that all three compounds acted as chicken AR antagonists and inhibit testosterone induced AR activation. qRT-PCR analysis demonstrated that the compounds also alter expression patterns of various genes.
The results of this study suggest that the investigated compounds are potential EDCs in chicken. Moreover, the study demonstrates that the combined use of in silico and in vitro techniques is a fast and reliable procedure to identify new toxic compounds in the environment.
The brominated flame retardants TBP-AE and TBP-DBPE antagonize the chicken androgen receptor and act as potential endocrine disrupters in chicken LMH cells
Per-Erik Olsson
Added on: 02-14-2024
[1] https://www.sciencedirect.com/science/article/abs/pii/S0887233315002040?via%3Dihub
