Non Animal Testing Database

Decompression sickness-on-a-chip

October 2023
University of Colorado Boulder, Boulder, USA
The aim of this study was to investigate the contribution of immune cells to decompression sickness (DCS), which can e.g, occur when divers ascent too rapidly to the surface. Therefore, the immune responses of peripheral blood mononuclear cells were measured within human lung-on–a-chip devices (obtained by Emulate) established with primary alveolar cells and microvascular cells. The devices were pressurized to 1.0 or 3.5 atm for 1 h and surrounded by normal air or oxygen-reduced air. Phenotyping of neutrophils, monocytes, and dendritic cells from whole blood as well as multiplexed ELISA revealed that immune responses occur within 1 hour and that normal air confer greater immune activation. This work strongly suggests innate immune cell reactions contribute to the aetiology of DCS. Moreover, the lung-on-a-chip offers a possibility for the screening of prophylactic drugs that can temper the immune reaction.
Dissolved gases from pressure changes in the lungs elicit an immune response in human peripheral blood
Charles Wyatt Shields
Added on: 12-02-2023
Back to Top
English German

Warning: Internet Explorer

The IE from MS no longer understands current scripting languages, the latest main version (version 11) is from 2013 and has not been further developed since 2015.

Our recommendation: Use only the latest versions of modern browsers, for example Google Chrome, Mozilla Firefox or Microsofrt Edge, because only this guarantees you sufficient protection against infections and the correct display of websites!