Investigation of microbiome effects on intestinal Candida overgrowth
2022
Leibniz Institute for Natural Product Research and Infection Biology - Hans-Knoell-Institute, Jena, Germany
Intestinal microbiota dysbiosis can initiate overgrowth of commensal Candida species, thereby leading to disseminated candidiasis.
In this study, a systems-biology approach involving multi-omics (transcriptome/metabolome) profiling, in silico metabolic modelling, and in vitro infection biology was used to uncover how Lactobacillus rhamnosus colonization of intestinal epithelial cells mediates protection against Candida albicans infection. Human colon cells were cultivated in monolayers and colonized with Lactobacillus rhamnosus for 18 h prior to infection with C. albicans. The results demonstrate that protection by L. rhamnosus colonization is a multifactorial process that synergistically affects C. albicans growth by reshaping the metabolic environment, forcing metabolic adaptations that reduce fungal pathogenicity. Moreover, the approach provides insights into how C. albicans pathogenicity can be controlled or prevented.
Lactobacillus rhamnosus colonisation antagonizes Candida albicans by forcing metabolic adaptations that compromise pathogenicity
Bernhard Hube
Added on: 09-25-2023
[1] https://www.nature.com/articles/s41467-022-30661-5
