In vitro investigation of metabolites for anti-doping analysis
2023
German Sport University Cologne, Cologne, Germany
The aim of this study was to investigate the metabolism of the anabolic agent RAD140 and compare different in vitro techniques to produce its metabolites.
Electrochemical (EC) oxidation, incubation with subcellular human liver fractions, and a 3D culture of human liver cells in an organ on a chip platform were used for the generation of metabolites of RAD140. The samples are analysed by means of liquid chromatography coupled to high-resolution tandem mass spectrometry. The resulting metabolite patterns were compared to that of a human doping control urine sample containing RAD140 and its metabolites.
A total of 16 metabolites were detected in urine, while 14, 13, and 7 metabolites were detected in samples obtained from the organ-on-a-chip experiment, the subcellular liver fraction, and EC experiments, respectively. In the organ on a chip samples, the highest number of metabolites were detected. The subcellular liver fractions and organ on a chip techniques are deemed complementary to predict metabolites of RAD140, as both techniques produce distinct metabolites that are also found in the human urine sample.
Comparison of in vitro approaches for predicting the metabolism of the selective androgen receptor modulator RAD140
Mario Thevis
Added on: 09-11-2023
[1] https://link.springer.com/article/10.1007/s00216-023-04835-z
