Brain metastasis-on-a-chip
October 2023
Seoul National University, Seoul, South Korea
The human brain choroid plexus (ChP) is a secretory tissue separating blood from the crerebrospinal fluid (CSF) thereby functioning as the blood-CSF barrier.
In this study, the ChP structure is recapitulated by developing a microfluidic chip which is used to culture human ChP cells. The brain extracellular matrix is mimicked by using a hydrogel and CSF flow dynamics by rocking the system. The use of the ChP-on-a-chip in drug screening is demonstrated by observing physiologically relevant drug responses from breast cancer cells that had spread in the ChP. ChP immune responses are also recapitulated, as demonstrated by the motility and cytotoxic effects of macrophages.
The human ChP-on-a-chip will facilitate the elucidation of ChP pathophysiology and support the development of therapeutics to treat cancers that have metastasized into the ChP.
Engineering choroid plexus-on-a-chip with oscillatory flow for modeling brain metastasis
Noo Li Jeon
Added on: 09-11-2023
[1] https://www.sciencedirect.com/science/article/pii/S2590006423002338?via%3Dihub
