A system for multicellular aggregates
December 2017
Department of Radiation Oncology, Medical Center – University of Freiburg, Freiburg, Germany
Spheroid use in oncological studies is limited by the capabilities of different cell types to self-assemble into 3D spheroids. This study describes a high-precision micro-molding technique that produces deep conical agarose microwell arrays that allow the cultivation of uniform multicellular aggregates, irrespective of the spheroid formation capacity of the cells. Such hydrogel arrays warrant a steady nutrient supply for several weeks, permit live volumetric measurements to monitor cell growth, enable immunohistochemical staining, fluorescence-based microscopy, and facilitate immediate harvesting of cell aggregates. The co-culture of a breast cancer cell line with bone marrow stromal cells is shown to enhance the 3D growth of the cancer cells in this system.
A deep conical agarose microwell array for adhesion independent three-dimensional cell culture and dynamic volume measurement
Per G. Lund, Andreas R. Thomsen
Added on: 04-21-2020
[1] https://pubs.rsc.org/en/content/articlelanding/2018/LC/C7LC00832E#!divAbstract[2] https://www.uniklinik-freiburg.de/presse/pressemitteilungen/archiv-2017/detailansicht/1345-universitaetsklinikum-freiburg-entwickelt-methode-zur-kultivierung-von-mini-tumoren.html
