Screening platform for assessment of NK cell cytotoxicity
2016
Karolinska Institutet, Tumor and Cell Biology, Stockholm, Sweden(1)
KTH – Royal Institute of Technology, Solna, Sweden(2)
KTH – Royal Institute of Technology, Solna, Sweden(2)
Cytotoxic effector lymphocytes, such as natural killer (NK) cells and T cells, are important for immune defence against cancer and viral infections, the traits that have made these cells valuable in adoptive cell therapy. This screening platform can be used for assessment of the cytotoxic potential of individual natural killer (NK) cells within larger populations. Human primary NK cells were distributed across a silicon–glass microchip containing a high number of individual microwells loaded with target cells. Through fluorescence screening and automated image analysis, the numbers of NK and live or dead target cells in each well could be assessed at different time points after initial mixing. Cytotoxicity was also studied by time-lapse live-cell imaging in microwells quantifying the killing potential of individual NK cells. Moreover, the screening approach was adapted to increase the chance to find and evaluate serial killing NK cells. This approach could find use in clinical applications, e.g., in the selection of donors for stem cell transplantation or generation of highly specific and cytotoxic cells for adoptive immunotherapy.
Microchip screening platform for single cell assessment of NK cell cytotoxicity
Björn Önfelt(1, 2)
Added on: 05-27-2020
[1] https://www.frontiersin.org/articles/10.3389/fimmu.2016.00119/full
