CRISPR gene editing can cause cell toxicity
2022
Barcelona institute for Science and Technology, Barcelona, Spain
In this study, scientists have found that depending on the targeted spot of the human genome, CRISPR gene editing can give rise to cell toxicity and genomic instability. Using computational methods, the team analysed the most popular CRISPR library designed for human cells and detected 3,300 targeted spots that show strong toxic effects. The study also reports that around 15 per cent of human genes contain at least one toxic editing point. These unwanted effects are mediated by the tumour suppressor protein p53 and are determined by the DNA sequence near the editing point and various epigenetic factors in the surrounding region.
TP53-dependent toxicity of CRISPR/Cas9 cuts is differential across genomic loci and can confound genetic screening
Fran Supek
Added on: 11-25-2022
[1] https://www.nature.com/articles/s41467-022-32285-1[2] https://www.drugtargetreview.com/news/104493/researchers-find-that-crispr-gene-editing-can-give-rise-to-cell-toxicity-and-genomic-instability/?utm_source=Email+marketing&utm_medium=email&utm_campaign=DTR+-+Newsletter+33+-+Bio-Rad+-+18.08.2022&utm_term=New+needle-free+nasal+vaccine+shows+promise+for+COVID-19&utm_content=https%3a%2f%2femails.drugtargetreview.com%2frussellpublishinglz%2f&gator_td=X1m9EyNpijNlcowWxOl30n4C75HvQ6r8vQIaziTemxJShKQT4wJq5FFro64B6rTEU%2bILd5ort8nsxbr3v1FwuLM3cEqzp%2fJckGtC4EvkS2Ix8Cw9ihfdr6vHYis6Wq6Vd0EVkU96QGGN81vKCQec1cvIp%2bTcOEsu1TkHm%2fqO7VPh3viKHSxGGYJ4ixnINrGFIM1Idf2y2DxGSG7KUK6jyWDFW03jz6QIMuAq7KGOz4Q%3d
