A DEP-array allows direct measurement of immune lysis
2013
Regina Elena National Cancer Institute, Immunology Laboratory, Rome, Italy
DEP arrays - chip platforms based on dielectrophoresis (DEP) - have the advantage that they allow direct measurement of cell lysis by identifying individual cells and capturing them in DEP "cages". "Cages" and their contents can be moved within the chip to any location on its surface. In this way, controlled and forced interactions between cells can be induced and infected cells and tumors can be detected. Here, DEP-based manipulations of human single CTL (Cytotoxic Lymphocyte) and NK (Natural Killer) cells as well as controlled and forced interactions were performed to understand and control lytic interactions. Specific lysis could be measured in real time and target cells with different susceptibility to immunolysis were identified. Applications are foreseeable in human immunology, including antiviral surveillance and tumor immunotherapy.
Lysis-on-Chip of single target cells following forced interaction with CTLs or NK cells on a dielectrophoresis-based array
Patrizio Giacomini
Added on: 05-27-2020
[1] https://www.jimmunol.org/content/191/7/3545