Influence of cancer cell secretion on cardiomyocytes
2022
University Medical Centre Mannheim, Mannheim, Germany
The aim of this study was to investigate the role of cancer cell secretion on cardiac dysfunction including arrhythmias, which appears frequently in patients with cancers. Human-induced pluripotent stem cell-derived cardiomyocytes generated from skin biopsies of three healthy donors were treated with media used for culturing gastrointestinal cancer (AGS and SW480) cells.
qPCR, patch-clamp experiments, western blotting, DNA methylation and demethylation analysis were performed. To prove that the observed changes of ion channel expression induced by cancer cell secretion were related to enhanced DNA methylation, methylation was reduced by overexpression of a demethylating enzyme (TET1). Gastrointestinal cancer cell secretion was shown to influence cardiac ion channel expression and function, which may contribute to the occurrence of arrhythmias in cancer patients.
Investigating the regulatory signalling, especially the epigenetic modifications of ion channels, could provide opportunities for discovering new therapeutic targets for treating arrhythmias in cancer patients.
Regulation of ion channel function in human-induced pluripotent stem cell-derived cardiomyocytes by cancer cell secretion through DNA methylation
Xiaobo Zhou
Added on: 10-27-2022
[1] https://www.frontiersin.org/articles/10.3389/fcvm.2022.839104/full
