In this study, RNA-sequencing (RNA-Seq) was used to analyse the following human in vitro liver cell models in comparison to healthy human liver tissue: cancer-derived cell lines, induced pluripotent stem cell-derived hepatocyte-like cells, human precision-cut liver slices, primary human hepatocytes and 3D liver microtissues. Baseline expression profile and gene regulatory networks were analysed and more comprehensive analyses using non-differentially expressed genes and differential transcript usage were applied. It was shown that 3D liver microtissues exhibited the highest similarity with in vivo liver at the beginning of the incubation period followed by a decrease during long-term incubation. Primary human hepatocytes also showed a high degree of similarity with human liver tissue and allowed stable conditions for a cultivation period of 24 h.
Comparing in vitro human liver models to in vivo human liver using RNA‑Seq
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