Lymph nodes (LN) are crucial locations where an immune response is intiated, yet it is difficult to replicate their structure and function in vitro. Here, a LN on-a-chip is constructed that resembles a human LN closely. The extracellular matrix (ECM) composition is comparabel, as well as its morphology, porosity, stiffness, and permeability. The immune cells reside in different compartments like in the human LN and a lymph fluid that flows through the device is diverted to a web like systems in a smilar flow pattern. The cells encapsulated in the hydrogel show typical viability and interaction among the cell types across chamber barriers can be observed. To test the device, hydroxychloroquine is used to investigate cell dynamics to this drug. The results suggest that the LN on-a-chip is a suitable 3D tool to study cellular dynamics, complex biological processes and drug-cell interactions.
Multi-compartment lymph-node-on-a-chip enables measurement of immune cell motility in response to drugs
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