Midbrain organoids for toxicity testing
2021
Max Planck Institute for Molecular Biomedicine, Muenster, Germany
In this study, an automated organoid model of the human midbrain was utilized to screen for the general neurotoxic and dopaminergic neuron-specific toxic effects of a library of 84 compounds including pesticides, drugs, flame retardants, and non-toxic controls. The toxic effects were evaluated on both, total cell viability and dopaminergic neurons. Follow-up dose-response experiments further confirmed the results of the primary screens. The method enables dissecting the toxicities of compound exposure to separate cellular subpopulations within human organoids at the single-cell level. Furthermore, the higher sensitivity in 3D culture was demonstrated in comparison to 2D cultures. The automated workflow is scalable and demonstrates the feasibility of quantitatively assessing cell-type-specific toxicity in human organoids in vitro.
Cell-type-specific high throughput toxicity testing in human midbrain organoids
Hans R. Schöler, Jan M. Bruder
Added on: 04-28-2022
[1] https://www.frontiersin.org/articles/10.3389/fnmol.2021.715054/full
