3D full-thickness skin-on-a-chip system
2022
Universidade Nova de Lisboa, Oeiras, Portugal
In this study, a 3D full-thickness skin-on-a-chip (SoC) system that reproduces key aspects of the in vivo cellular microenvironment is presented. The approach combines the production of a fibroblast-derived matrix with the use of an inert porous scaffold for the long-term, stable cultivation of a human skin model. The developed SoC includes a fully differentiated epidermal compartment with increased thickness and barrier function. Contrary to other SoC platforms that include a collagen-based matrix, the described model presents superior stability and physiological relevance. The skin barrier function was quantitatively evaluated using integrated transepithelial electrical resistance sensors offering a non-destructive real-time tool to study the skin barrier function. In the future, this innovative low-cost platform could provide a new in vitro tissue system compatible with long-term studies to study skin diseases and evaluate the safety and efficacy of novel drugs and technologies.
Biomimetic full-thickness skin-on-a-chip based on a fibroblast-derived matrix
Abel Gonzalez Oliva
Added on: 03-31-2022
[1] https://www.mdpi.com/2673-8023/2/1/13
