3D multicellular model of tumor microenvironment
2016
University of Freiburg, Freiburg, Germany
In recent years, 3D models are becoming increasingly important in drug screening experiments due to their capacity of reproducing key physiological aspects impossible to reproduce in 2D cultures and the possibilities to avoid the complexity of working with animal models. Here, a 3D multicellular spheroid model was built using human lung epithelial cancer cells, human pulmonary vascular cells and human marrow-derived mesenchymal stem cells to reproduce epithelial tumor microenvironment. The results showed that it was possible to observe key characteristics of the in vivo disease that other spheroid cultures fail to reproduce. Moreover, despite having elevated levels of reactive oxygen species and expression of genes associated with drug resistance, cells within the model were more sensitive to paclitaxel and gemcitabine than 2D tri-cultures. Overall, the researchers propose a new 3D spheroid model that contains different cell types and is able to replicate key aspects of cancer pathology, making it a potential tool to perform drug screening and mechanistic studies.
Recapitulating epithelial tumor microenvironment in vitro using three dimensional tri-culture of human epithelial, endothelial, and mesenchymal cells
V Prasad Shastri
Added on: 11-28-2021
[1] https://bmccancer.biomedcentral.com/articles/10.1186/s12885-016-2634-1[2] https://data.jrc.ec.europa.eu/dataset/176d71e6-5082-4b29-8472-b719f6bda323
