Organoids for modelling Zika virus exposure
2016
Johns Hopkins University School of Medicine, Baltimore, USA
The authors developed a miniaturized spinning bioreactor (SpinΩ) to generate forebrain-specific organoids from human iPSCs that recapitulate key features of human cortical development, notably including a distinct human-specific outer radial glia cell layer, which is absent in other animals. The authors also developed protocols for midbrain and hypothalamic organoids. Finally, the forebrain organoid platform could successfully be used to model Zika virus exposure, infection and resulting microcephaly. Therefore, this versatile platform is suitable for modelling human brain development and disease and for compound testing, including potential Zika antiviral drugs.
Brain-region-specific organoids using mini-bioreactors for modeling ZIKV exposure.
Guo-li Ming, Hongjun Song
Added on: 05-25-2020
[1] https://www.cell.com/cell/fulltext/S0092-8674(16)30467-6?_returnURL=https%3A%2F%2Flinkinghub.elsevier.com%2Fretrieve%2Fpii%2FS0092867416304676%3Fshowall%3Dtrue
