3D model to assess exposure of lung tissue to cigarette smoke
Company 2015
Philip Morris Products S.A., Neuchâtel, Switzerland
Cigarette smoke has been consistently associated with the development of lung diseases. However, there are severe limitations in epidemiological studies due to the difficulties in accessing tissue samples. Culture models have been also used to evaluate the impact of cigarette smoke on human bronchial epithelial cells, but the exposure to the toxic compounds is different from the in vivo situation. Recent 3D models allow a better approach because they can be cultured at the air-liquid interface and can contain different cell types. Here, an in vitro exposure platform is developed with organotypic cultures of human bronchial and nasal tissue to assess the effects of cigarette smoke on human lung tissue. The results showed increased CYP1A1/1B1 activity after exposure to cigarette smoke. Furthermore, exposed cultures also had alterations related to xenobiotic metabolism similar to those seen in human smokers' tissue samples. Overall, the researchers develop a consistent model to study the changes induced by cigarette smoke exposure in human lung tissue.
Impact assessment of repeated exposure of organotypic 3D bronchial and nasal tissue culture models to whole cigarette smoke
Stephanie Boue
Added on: 11-22-2021
[1] https://www.jove.com/t/52325/impact-assessment-repeated-exposure-organotypic-3d-bronchial-nasal[2] https://data.jrc.ec.europa.eu/dataset/176d71e6-5082-4b29-8472-b719f6bda323
