Hyaluronan scaffolds for 3D lung tumor spheroids
2018
University of Aveiro, Aveiro, Portugal
In recent years, there is a growing interest in 3D multicellular tumor spheroids that can reproduce complex tumor microenvironments in vitro. However, in vitro 3D lung tumor models that faithfully reproduce their in vivo counterparts are still lacking. Here, a multicellular 3D model was developed co-culturing human lung adenocarcinoma cell line, human fibroblasts and human bone-marrow-derived-mesenchymal stem cells with bioinstructive hyaluronan microparticles that mimic the tumor extracellular matrix to improve lung tumor microenvironment modelling. The results showed that the 3D microspheres increased cell viability and self-assemble together with CD44 expression. These 3D cultures were reproducible and presented characteristics of in vivo solid tumors. Moreover, mesenchymal stem cells exhibited human tumor-like behaviour by migrating through the 3D spheres and interacting with cancer cells and fibroblasts. Finally, administration of doxorubicin in cultures with mesenchymal stem cells revealed their cytotoxic responses, showing the critical need of including mesenchymal stem cells and fibroblasts in 3D in vitro lung tumor models. Overall, the researchers provide evidence of the importance of combining different cell types in tumor models and demonstrate that hyaluronan-microparticles can be used as tumor spheroids scaffolds with the potential to combine them with different extracellular matrix components to improve 3D models.
Bioinstructive microparticles for self-assembly of mesenchymal stem cell-3D tumor spheroids
J F Mano, V M Gaspar
Added on: 11-22-2021
[1] https://www.sciencedirect.com/science/article/abs/pii/S0142961218306380[2] https://data.jrc.ec.europa.eu/dataset/176d71e6-5082-4b29-8472-b719f6bda323
