Influence of culture substrates in cancer cell biology
December 2017
Trinity College Dublin, Dublin, Ireland
3D in vitro models are increasingly used to reproduce key features of cancer biology in drug discovery. However, culture substrates remain poorly characterized, and it is still unknown how they influence cancer cell responses to drug testing. Here, 2D and 3D cultures of human adenocarcinoma cells are used to evaluate the influence of different substrates in the morphological, mechanical and biochemical properties of the cells. The results offer a proper evaluation of cell features when growing on soft 3D substrates or two-dimensional glass surfaces. Moreover, drug response to docetaxel was evaluated in different culture conditions. Overall, this study provides a deep characterization of a human adenocarcinoma cell line and the potential of its use in 3D systems for lung cancer research.
Culturing substrates influence the morphological, mechanical and biochemical features of lung adenocarcinoma cells cultured in 2D or 3D
Dania Movia
Added on: 11-22-2021
[1] https://www.sciencedirect.com/science/article/abs/pii/S0040816617303129[2] https://data.jrc.ec.europa.eu/dataset/176d71e6-5082-4b29-8472-b719f6bda323
