3D models are emerging as dynamic models with high value to study cell growth and proliferation. Nowadays, coupled 3D modelling and imaging techniques offer easy-to-use systems. To study anti-cancer drugs, a simple visualization setup to image non-proliferative cells is needed. Here, a fluorescent-based assay is used to analyze cell proliferation of human breast cancer cells in a 3D model using a pulse-labelling technique with protein Kaede. The results show that it was possible to analyze the effects of 5-fluorouracil and PD0332991 anti-cancer drugs in human breast cancer cells. Overall, the researchers present a newly developed platform to easily study anti-cancer drug effects on cell proliferation to be able to do fast drug screening assays in human cells cultured in 3D conditions.
Data of a fluorescent imaging-based analysis of anti-cancer drug effects on three-dimensional cultures of breast cancer cells
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