Impact of modified proteins in multiple sclerosis studied using patients' samples
2019
University Hospital Zurich, Zürich, Switzerland
In Multiple Sclerosis (MS), a chronic inflammatory autoimmune disease of the central nervous system (CNS), the amount of the citrullinated proteins is increased in white matter. In the present study, the researchers investigated the presence of citrulline in proteins of brain tissues from MS patients and controls. Then, the researchers assessed in vitro the immune response of T cells isolated from donors to citrullinated and unmodified proteins. The study establishes a map of citrullinated proteins in human brain tissue and shows that the number of modified proteins in MS white matter was higher than in control tissue. The approach developed here allows a deep analysis of the proteome of small tissue samples and the testing of the impact of modified proteins in the immune response.
Brain citrullination patterns and T cell reactivity of cerebrospinal fluid-derived CD4+ T cells in multiple sclerosis
Roland Martin
Added on: 10-29-2021
[1] https://www.frontiersin.org/articles/10.3389/fimmu.2019.00540/full[2] https://data.jrc.ec.europa.eu/dataset/700397b2-edd7-4ed6-86f7-fc1b164ed432
