Breast fibroblasts induce breast cancer stem cell transformation
2018
Capital Medical University, Beijing, China
Breast cancer stem cells have an important role in metastasis and, recently, it has been shown that they have different phenotypes in different tumour areas at different stages of cancer progression. Cancer-associated fibroblasts have been described to induce epithelial-to-mesenchymal transition, thus promoting cancer stem cell features and their phenotypical transformation. However, the role of normal fibroblasts in breast cancer stem cell behaviour remains unknown. Here, cancer-associated and normal fibroblasts from patients were analyzed and used to test the effects of their paracrine activity on breast cancer stem cells. The results showed that there are no major morphological differences among both types of fibroblasts, but they have different proteomic profiles and cell invasion capacities. Furthermore, cancer-associated fibroblasts conditioned media was more efficient in promoting mammosphere formation than that of normal fibroblasts and both media generated different marker phenotypes of breast cancer stem cells. Overall, the researchers confirmed the differences among cancer-associated and normal fibroblasts and further elucidated their ability to generate different phenotypes of breast cancer stem cells.
Breast fibroblasts in both cancer and normal tissues induce phenotypic transformation of breast cancer stem cells: a preliminary study
Hua Kang
Added on: 10-27-2021
[1] https://peerj.com/articles/4805/[2] https://data.jrc.ec.europa.eu/dataset/ffebe454-ed9a-47cf-8a33-8cf70c1b7d38
