3D model to study drug resistance in cancer
2018
Dalian Medical University, Dalian, China
Multidrug resistance is a very important limitation of chemotherapy that hinders the efficiency of current treatments. However, there is not enough data generated that can be useful to understand and overcome this complication due to the complexity of recapitulating drug-resistant cancer cells behaviour in vitro. Here, a 3D model is developed culturing adriamycin-resistant human breast cancer cells in silk-collagen scaffolds to better replicate in vivo features. The results showed that breast cancer cells behaved differently in 2D versus 3D cultures, involving cancer and drug resistance-related mechanisms. Additionally, cancer cells were more resistant to drugs in the 3D culture through increased drug efflux capability. Moreover, cells presented an altered cell cycle distribution and there was an increased proportion of breast cancer stem cells, which might have a critical role in the development of drug resistance in tumours. Overall, the researchers develop a robust 3D model that better replicates drug-resistant cancer cell behaviour, which may allow to better investigate the mechanisms underlying the development of cancer drug resistance and further improve chemotherapeutic strategies.
Three-dimensional tissue culture model of human breast cancer for the evaluation of multidrug resistance
Xiuli Wang
Added on: 10-26-2021
[1] https://onlinelibrary.wiley.com/doi/10.1002/term.2729[2] https://data.jrc.ec.europa.eu/dataset/ffebe454-ed9a-47cf-8a33-8cf70c1b7d38
