Proteomic analysis of breast cancer-associated fibroblasts
2014
University of Vienna, Vienna, Austria
Breast cancer is the most prevalent form of cancer in women. Among all the actors involved in cancer progression, cancer-associated fibroblasts have been described to have a pivotal role. However, this type of cells has not yet been properly characterized. Here, patients' samples from different areas of the tumor and its surroundings were used to perform a proteomic analysis and subsequently compared to reference profiles from activated and non-activated primary human mammary fibroblasts and a human carcinoma cell line. The results showed that, within the tumor, there is a prevalence of cancer cell-related proteins, while in distant areas there is a predominance of fibroblast-related proteins. Furthermore, several proteins related to fibroblast wound healing activity were found in all tissue samples, contrary to inflammatory-related proteins. Overall, the authors of this study establish a proteomic profile of cancer-associated fibroblasts present in different areas of the cancerous wound, which could be used to better understand this cell type and develop new therapeutical strategies based on its modulation.
Proteome profiling of breast cancer biopsies reveals a wound healing signature of cancer-associated fibroblasts
Christopher Gerner
Added on: 10-21-2021
[1] https://pubs.acs.org/doi/abs/10.1021/pr500727h[2] https://data.jrc.ec.europa.eu/dataset/ffebe454-ed9a-47cf-8a33-8cf70c1b7d38
