Spheroids to study cancer stem cells
2018
Ankara University, Ankara, Turkey
Cancer stem cells have become a popular subject of study. To better understand their properties, it is necessary to improve the current in vitro models. Here, a 3D in vitro system is used to analyse the cancer stemness of a human breast cancer cell line and a human glioblastoma cell line. The results showed that breast cancer cells were able to form uniform 3D structures and acquired cell stem cell features. Targeting cancer stem cells with metformin reduced the expression of genes related to drug resistance. Furthermore, it also increased the effectiveness of 5-fluorouracil. Overall, the researchers demonstrate that the use of 3D models in vitro can improve the translationality of cancer models, as they help replicate tumour microenvironments and have diverse cellular populations as it occurs in vivo.
Evaluation of cancer stemness in breast cancer and glioblastoma spheroids in vitro
Açelya Yilmazer
Added on: 10-18-2021
[1] https://link.springer.com/article/10.1007%2Fs13205-018-1412-y[2] https://data.jrc.ec.europa.eu/dataset/ffebe454-ed9a-47cf-8a33-8cf70c1b7d38
