Primary cultures are a basic tool in neuroscience. However, most of the time these cultures are done with rodent brains, associated with several limitations in the translational potential of the model. In this study, human fetal brains are used to produce mixed primary brain cultures. The results show characteristic features in human cultures not present in animal-based models. With the proper methods, it is possible to maintain brain cells for a long time in culture, producing different types of neurons and glia. Additionally, consistent production of amyloid-beta, tau and other characteristic markers of Alzheimer's disease is also described. Basically, the study presents a new human model with a detailed characterization that can be a powerful tool to enhance the translationality of neuroscience studies and help to better understand the mechanisms of human brain disorders, leading to new therapeutic approaches.
Human primary mixed brain cultures: preparation, differentiation, characterization and application to neuroscience research
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