Study of hundreds of proteins in parallel
October 2020
CeMM Research Center for Molecular Medicine of the Austrian Academy of Sciences, Vienna, Austria
The authors describe a scalable strategy to characterize effects on protein localization and levels in response to different perturbations. The study utilizes CRISPR-Cas9-based intron tagging to generate cell pools expressing hundreds of GFP-fusion proteins from their endogenous promoters and monitor localization changes by time-lapse microscopy followed by clone identification using in situ sequencing. This strategy can characterize cellular responses to drug treatment and thus, identify nonclassical effects such as modulation of protein-protein interactions, condensate formation, and chemical degradation.
Pooled protein tagging, cellular imaging, and in situ sequencing for monitoring drug action in real time
Stefan Kubicek
Added on: 12-17-2020
[1] https://genome.cshlp.org/content/30/12/1846.full[2] https://www.bionity.com/en/news/1168753/study-of-hundreds-of-proteins-in-parallel.html
